| ApplicationReferences | ParaffinSectionsPezzella,F.,etal.1994.J.Clin.Pathol.47,592.OriginalCloneGannon,J.V.,etal.1990.EMBOJ.9,1595.EpitopeIdentificationStephen,C.W.andLane,D.P.,1992.J.Mol.Biol.225,1.ImmunofluorescenceBernardi,R.,etal.2004.Nat.CellBiol.6,665. | 
| KeyApplications | Immunoblotting(WesternBlotting) Immunofluorescence
 Immunoprecipitation
 ParaffinSections
 FlowCytometry
 FrozenSections
 GelShift
 
 | 
| ApplicationNotes | FlowCytometry(1-20μg/ml) FrozenSections(10μg/ml)
 GelShift(seecomments)
 Immunoblotting(5μg/ml)
 Immunofluorescence(1-20μg/ml,seeapplicationreferences)
 Immunoprecipitation(1μgpersample)
 ParaffinSections(seeapplicationreferences)
 | 
| ApplicationComments | Undernon-denaturingconditions(immunoprecipitation,immunofluorescenceandfrozensections),Anti-p53(Ab-3)doesnotrecognizenormal(wild-type)p53protein;itrecognizesanepitopeexposedbyactivatingmutationsordenaturation.Indenaturingprotocols(immunoblottingandparaffinsections),Anti-p53(Ab-3)willrecognizebothmutantandwild-typep53.Willnotrecognizetosomep53moleculeswithmutationsintheRHSVVepitope,butwillreacttoTFIIIA,whichhastheRHSVVepitope.Forgelshiftassay,useCat.No.OP29LandresUSPendin100μlbuffer.Antibodyshouldbetitratedforoptimalresultsinindividualsystems. |