| Immunogen | TheantibodyepitopewasmappedtoasitewithinaconsensustyrosinesulfationmotifofPSGL-1,previouslyshowntobeessentialforinteractionwithP-selectin(andnowshowntobeessentialforrecognitionofPSGL-1byL-selectin). | 
| Clone | KPL-1 | 
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. | 
| Host | Mouse | 
| Specificity | P-selectinglycoproteinligand-1(PSGL-1).TheantibodyepitopewasmappedtoasitewithinaconsensustyrosinesulfationmotifofPSGL-1,previouslyshowntobeessentialforinteractionwithP-selectin(andnowshowntobeessentialforrecognitionofPSGL-1byL-selectin).{SnappKRetal.1998}.ThebindingofKPL-1tohumanPSGL-1onHL-60cellsdoesnotrequiresulfation{Thatte,Aetal2002}. | 
| Isotype | IgG1κ | 
| SpeciesReactivity |  | 
| SpeciesReactivityNote | KnownnottoreactwithRat,Bovine,Pig,andEquinePSGL-1{Snapp,KRetal1998;Baisse,Betal2007}. | 
| AntibodyType | MonoclonalAntibody | 
| EntrezGeneNumber |  | 
| EntrezGeneSummary | SELPLGisthehighaffinitycounter-receptorforP-selectinonmyeloidcellsandstimulatedTlymphocytes.Assuch,itplaysacriticalroleinthetetheringofthesecellstoactivatedplateletsorendotheliaexpressingP-selectin.TheorganizationoftheSELPGgenecloselyresemblesthatofCD43andthehumanplateletglycoproteinGpIb-alphabothofwhichhaveanintroninthe5-prime-noncodingregion,alongsecondexoncontainingthecompletecodingregion,andTATA-lesspromoters. | 
| GeneSymbol | SELPLGCLAPSGL-1CD162PSGL1
 | 
| UniProtNumber |  | 
| UniProtSummary | FUNCTION:SwissProt:Q14242#BindstoP-,E-andL-selectins.Thecalcium-dependenthighaffinityinteractionwithP-selectinmediatesthetetheringandrollingofneutrophilsandT-lymphocytesonendothelialcells. SIZE:412aminoacids;43201Da
 SUBUNIT:Homodimer;disulfide-linked.
 SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
 TISSUESPECIFICITY:Expressedonneutrophils,monocytesandmostlymphocytes.
 PTM:Displayscomplex,sialylatedandfucosylatedO-linkedoligosaccharides,atleastsomeofwhichappeartocontainpoly-N-acetyllactosaminewithvaryingdegreesofsubstitution.AlsoN-glycosylated.&SulfatedintheN-terminalregion;sulfationisnecessaryforP-selectinbinding.
 |