| Immunogen | Bovinebrainmicrotubuleprotein. | 
| Clone | AP20 | 
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. | 
| Host | Mouse | 
| Specificity | RecognizesMAP-2(microtubuleassociatedprotein-2).MAP-2isoneofseveralhighmolecularweightproteinsthatplayanimportantroleinbrainmicrotubuleassembly.Inadditiontoitsassociationwithmicrotubules,MAP-2associateswithneurofilamentsandactinfilamentssuggestingthatitmayguideinteractionamongmicrotubules,othercytoskeletalelements,andcytoplasmicorganelles(1). 
 MAP-2isastringentMarkerforneurons.Inaddition,MAP-2displaysintracellularspecificity.Inthecentralnervoussystem,MAP-2isconfinedtoneuronalcellbodiesanddendrites.Thereareexceptions,however,wheresomeaxonsstainpositiveforsmallamountsofMAP-2(2,3).MAP-2isuniformlydistributedthroughoutthecellwhenfirstexpressedinculturedneuronsbutbecomesselectivelylocalizedasdendriticdevelopmentproceeds(4,5).
 
 SDS-PAGEProfiles:InSDS-PAGEMAP-2fromadultratmigratesasacloselyassociateddoublethavingamolecularweightofapproximately300kD.However,earlyinbraindevelopment(postnatalday10inrats),MAP-2migratesasasinglebandthatisidenticaltothelowermolecularweightbandoftheadultMAP-2doublet(MAP-2b).Laterindevelopment(postnataldays17-18),themobilityofMAP-2changestotheadultdoubletform.(Inthespinalcord,conversiontotheadultformoccursearlier).
 | 
| Isotype | IgG1 | 
| SpeciesReactivity | BovineChickenHumanMouseRat
 | 
| AntibodyType | MonoclonalAntibody | 
| EntrezGeneNumber |  | 
| EntrezGeneSummary | Thisgeneencodesaproteinthatbelongstothemicrotubule-associatedproteinfamily.Theproteinsofthisfamilyarethoughttobeinvolvedinmicrotubuleassembly,whichisanessentialstepinneurogenesis.Theproductsofsimilargenesinratandmouseareneuron-specificcytoskeletalproteinsthatareenrichedindentrites,implicatingaroleindeterminingandstabilizingdentriticshapeduringneurondevelopment.Anumberofalternativelysplicedvariantsencodingdistinctisformshavebeendescribed. | 
| GeneSymbol | MAP2MAP2BMAP-2MAP2CDKFZp686I2148MAP2A
 | 
| PurificationMethod | ProteinAPurfied | 
| UniProtNumber |  | 
| UniProtSummary | FUNCTION:SwissProt:P11137#TheexactfunctionofMAP2isunknownbutMAPsmaystabilizethemicrotubulesagainstdepolymerization.Theyalsoseemtohaveastiffeningeffectonmicrotubules. SIZE:1827aminoacids;199539Da
 PTM:MAP2A/cisphosphorylated.PhosphorylateduponDNAdamage,probablybyATMorATR.
 SIMILARITY:SwissProt:P11137##Contains3Tau/MAPrepeats.
 |