| ApplicationNotes | AntibodyispredilutedandreadytouseforImmunohistochemistryofformalin-fixed,paraffin-embeddedtissues. 
 Pretreatment:HeatInducedEpitopeRetrieval(HIER).RecommendEDTA,pH8.0(Cat.No.21546).NosignalwasdetectedwithoutEpitoperetrieval.
 
 Incubation:10minuteswithIHCSelect?DetectionKits.
 
 CD4hasbeenpredilutedforuseastheprimaryantibodywithChemicon"sIHCSelect?DetectionKitsandProtocols(CatalogNos.DAB050,DET-HP1000,APR050,andDET-APR1000),butothersupplier"sIHCdetectionsystemsmaybeused.Foroptimizedprotocoldetails,visitwww.chemicon.comandselecttheprotocolslinkunderCat.No.IHCR2110-6.
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