| Components | Positive/NegativeControlSlides,NaturalboxNote:2slides,eachcontainingamixturepositiveandnegativecontrolcells.BlockingBuffer,WhitecapH2O2/UreaTablets,AmbervialProteinaseK,PinkcapDABTablets,AmbervialTdTEnzyme,YellowcapBr-Dutp,Violetcap200XStreptavidin-HRPConjugate,Blackcap5XReactionBuffer,GreencapBiotin-labeledBrdUmAb,OrangecapMethylGreensolution,Naturalcap,TheAPO-BRDU-IHC?Kitisshippedinonecontainerandshouldbestoredat-20°Cuponarrival.Note:Afterthekitisdefrostedbeforefirstuse,boththe5XReactionBufferandtheBiotin-labeledanti-BrdUantibodyshouldbestoredat2-8°C.Also,theMethylGreensolutionshouldbestoredatroomtemperature.DONOTRE-FREEZEanyofthesecomponentsoncethawed.Reagentbottleshavecolorcodedcapstoaidintheiridentification.Sufficientreagentsareprovidedtoprocess50slidesincluding2positive/negativecontrolslides.Thecontrolcellsarederivedfromahumanpromyelociticleukemiacellline(HL60)andincubatedwith0.5mg/mLactinomycinDfor19hourstoinduceapoptosis.
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