| Immunogen | Syntheticpeptidecorrespondingtoaminoacids41-56ofhumancaspase-9(Duanetal.1996;Srinivasulaetal.1996;Nijhawanetal.1997).Recognizesproform(46kDa);sequenceanalysispredictscross-reactivitywithmousecaspase-9(>90%). | 
| Epitope | proform&lg.subunit | 
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. | 
| Host | Rabbit | 
| Specificity | Apoptosisisrelatedtomanydiseasesandinducedbyafamilyofcelldeathreceptorsandtheirligands.Celldeathsignalsaretransducedbydeathdomaincontainingadaptermoleculesandmembersofthecaspasefamilyofproteases.AnovelmemberinthecaspasefamilywasrecentlyidentifiedanddesignatedICE-LAP6,Mch6,andApaf-3(Duanetal.1996;Srinivasulaetal.1996;Nijhawanetal.1997).Caspase-9andApaf-1bindtoeachother,whichleadstocaspase-9activation(Nijhawanetal.1997).Caspase-9isalsoactivatedbygranzymeBandCPP32(Duanetal.1996;Srinivasulaetal.1996).Activatedcaspase-9cleavesandactivatescaspase-3thatisoneofthekeyproteases,beingresponsIBLefortheproteolyticcleavageofmanykeyproteinsinapoptosis(Nijhawanetal.1997).Caspase-9playsacentralroleincelldeathinducedbyawidevarietyofapoptosisinducersincludingTNF-alpha,TRAIL,anti-CD-95,FADD,andTRADD(Panetal.1998).Caspase-9isexpressedinavarietyofhumantissues(Duanetal.1996;Srinivasulaetal.1996). | 
| SpeciesReactivity |  | 
| AntibodyType | PolyclonalAntibody | 
| EntrezGeneNumber |  | 
| EntrezGeneSummary | Thisgeneencodesamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.ThisproteinisprocessedbycaspaseAPAF1;thisstepisthoughttobeoneoftheearliestinthecaspaseactivationcascade.Alternativesplicingresultsintwotranscriptvariantswhichencodedifferentisoforms. | 
| GeneSymbol | - CASP9
 - CASP-9
 - ICE-LAP6
 - APAF3
 - MCH6
 - CASPASE-9c
 - OTTHUMP00000044594
 - APAF-3
 - EC3.4.22.62[Contains:Caspase-9subunitp35
 - Caspase-9subunitp10].
 
  | 
| Non-ReactiveSpecies |  | 
| UniProtNumber |  | 
| UniProtSummary | FUNCTION:SwissProt:P55211#Isoform2lacksactivityisandominant-negativeinhibitorofcaspase-9. SIZE:416aminoacids;46281Da SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya35kDa(p35)anda10kDa(p10)subunit.Caspase-9andAPAF1bindtoeachotherviatheirrespectiveNH2-terminalCED-3homologousdomainsinthepresenceofcytochromeCandATP.InteractswiththeinhibitorsBIRC2,BIRC4,BIRC5andBIRC7. TISSUESPECIFICITY:Ubiquitous,withhighestexpressionintheheart,moderateexpressioninliver,skeletalmuscle,andpancreas.Lowlevelsinallothertissues. PTM:CleavagesatAsp-315bygranzymeBandatAsp-330bycaspase-3generatethetwoactivesubunits.Caspase-8and-10canalsobeinvolvedintheseprocessingevents. SIMILARITY:SwissProt:P55211##BelongstothepeptidaseC14family.&Contains1CARDdomain. |