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Millipore/AB1542 | Anti-Tyrosine Hydroxylase Antibody/AB1542/100 µL
  • Millipore/AB1542 | Anti-Tyrosine Hydroxylase Antibody/AB1542/100 µL

Millipore/AB1542 | Anti-Tyrosine Hydroxylase Antibody/AB1542/100 µL

價(jià)格: ￥4188.00 市場(chǎng)價(jià): 6980.00
貨號(hào): AB1542
品牌: Millipore
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    • Description
      CatalogueNumberAB1542
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-TyrosineHydroxylaseAntibody
      AlternateNames
      • Tyrosine3-hydroxylase
      • Tyrosine3-hydroxylase
      • tyrosinehydroxylase
      BackgroundInformationTyrosinehydroxylaseplaysanimportantroleinthephysiologyofadrenergicneurons.Itisthefirstandrate-limitingenzymeinvolvedinthebiosynthesisofthecatecholaminesDopamineandNorepinephrinefromtyrosine.THis,therefore,ausefulMarkerfordopaminergicandnoradrenergicneurons.TheenzymaticactivityofTHrequiresferrousionsascofactorsandisbelievedtoberegulatedbyphosphorylation.AtleastfourisoformsofhumanTHhavebeenidentifiedwhichresultfromalternativesplicing.
      ProductInformation
      FormatAffinityPurified
      Control
      • Positive:brain(corpusstriatum)andadrenalgland.Negative:liver.
      PresentationPurifiedsheeppolyclonalinbuffercontaining10mMHEPES,pH7.5,150mMNaCl,100μg/mLBSAand50%glycerol.
      StorageandShippingInformation
      StorageConditionsStablefor6monthsat-20to-80°Cinundilutedaliquotsfromdateofreceipt.StoreinaNON-self-defrostingfreezer.
      HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIllityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationAnti-TyrosineHydroxylaseAntibodyisanantibodyagainstTyrosineHydroxylaseforuseinIH&WB.
      KeyApplications
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesImmunohistochemistry(peroxidase):Apreviouslotofthisantibodywasusedat1:1,000dilution.Theantibodygivesspecificlabelingofnoradrenergicaxonsinprimatecerebralcortex(BrainRes.,1989,500:313-324.).
      Note:ItisimportantthatyouuseasecondaryantibodythathassignificantreactivitytosheepIgG1sincethemajorityofthesheepimmunoglobulinisIgG1.
      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenNativetyrosinehydroxylasefromratpheochromocytoma(J.Biol.Chem.,1982,257:9416-9423).
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostSheep
      SpecificityTyrosineHydroxylase(TH,TyrosineMonooxygenase).Cross-reactswithallmammalianformstestedtodateandsomenon-mammalianforms.
      SpeciesReactivity
      • Mammals
      • Rat
      • Mouse
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTyrosinehydroxylaseisinvolvedintheconversionoftyrosinetodopamine.Astherate-limitingenzymeinthesynthesisofcatecholamines,tyrosinehydroxylasehasakeyroleinthephysiologyofadrenergicneurons.MutationsinthisgenehavebeenassociatedwithautosomalrecessiveSegawasyndrome.Alternativelysplicedtranscriptvariantsencodingdifferentisoformshavebeennotedforthisgene.[providedbyRefSeq].

      TranscriptVariant:Thisvariant(2)usesadifferentdonorsplicesiteatthefirstcodingexonandismissinganadjacentin-framecodingexoncomparedtotranscriptvariant1,resultinginanisoform(b)missinga31aasegmentcomparedtoisoforma.
      GeneSymbol
      • TH
      • TYH
      • EC1.14.16.2
      PurificationMethodImmunoAffinityPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P07101#Playsanimportantroleinthephysiologyofadrenergicneurons.
      COFACTOR:Fe(2+)ion.
      SIZE:528aminoacids;58524Da
      TISSUESPECIFICITY:Mainlyexpressedinthebrainandadrenalglands.
      DISEASE:SwissProt:P07101#DefectsinTHarethecauseofautosomalrecessiveSegawasyndrome[MIM:605407];alsoknownasDOPA-responsivedystonia.Typically,itbeginsinchildhoodoradolescencewithprogressivedifficultyinwalkingand,insomecases,spasticity.Somecasespresentwithparkinsoniansymptomsininfancyandarereferredtoasautosomalrecessiveinfantileparkinsonism.
      SIMILARITY:SwissProt:P07101##Belongstothebiopterin-dependentaromaticaminoacidhydroxylasefamily.
      MolecularWeight~60kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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