| ApplicationNotes | Immunohistochemistryat1:500-1:1,000. 
 PERFUSIONMIXTUREANDPROTOCOLFORIMMUNOCYTOCHEMICALDETECTIONOFDOPAMINE:Seesuggestedprotocols.
 
 AB122Sshouldbeusedatdilutionsof1:500-1:1,000usingTris-MBScontaining0.5%Tritionand1%normalgoatserum.Theantibodyshouldbeincubated15hoursat+4°Corroomtemperature.Finalworkingdilutionsmustbedeterminedbyenduser.
 
 PostTreatment:
 
 TheimmunocytochemistryprocedureisthencontinuedwiththePAPmethodortheavidin-biotinperoxidasesystem.Note:sinceMBSisknowntoinhibitPAPoravidin-biotinperoxidase,itshouldbeomittedforthisstep.40mMTris-HClwith9%NaCladjustedtopH7.6(usingconcentratedHCl)shouldbeused.
 
 Notations:
 
 MBSisessentialinthepre-washandtheglutaraldehydesolutionsincethisantioxidantprotectsthedopaminemoleculefrombeingreleased.
 
 *Protocolmodifiedfrom:McRae-Degueurce,A.,etal.(1986).BrainResearch376:217-219.
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