| CatalogueNumber | 2760 | 
| BrandFamily | Chemicon® 
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| TradeName |  | 
| Description | BrdUIHCKit | 
| Overview | TestPrinciple 
 CHEMICON?"sBrdUImmunohistochemistryKitisanon-isotopicimmuno-histochemistrystainingkitforthedetectionandlocalizationofBrdUincorporatedintonewlysynthesizedDNAofproliferatingcells.VisualstainingofthecellsisachievedbyusingabiotinylatedmonoclonalantibodytoBrdUfollowedbystreptavidin-HRPconjugateandDABsubstrate.
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| BackgroundInformation | Evaluationofcellcycleprogressionisessentialforinvestigationsinmanyscientificfields.Measurementof[3H]thymidineincorporationascellsenterSphasehasbeenatrADItionalmethodfordetectionofcellproliferation.Subsequentquantificationof[3H]thymidineisperformedbyscintillationcountingofautoradiography.Thistechnologyisslow,laborintensiveandhasseverallimitationsincludingthehandlinganddisposalofradioisotopesandthenecessityofexpensiveequipment. 
 Awellestablishedalternativeto[3H]thymidineuptakehasbeendemonstratedbynumerousresearchers.Inthesemethodsbromodeoxyuridine(BrdU),athymidineanalog,replaces[3H]thymidine.BrdUisincorporatedintonewlysynthesizedDNAstrandsofactivelyproliferatingcells.FollowingpartialdenaturationofdoublestrandedDNA,BrdUisdetectedimmunochemicallyallowingtheassessmentofthepopulationofcellswhicharesynthesizingDNA.
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| MaterialsRequiredbutNotDelivered | 1.QuenchingSolution:Hydrogenperoxide(30%)solutionforquenchingendogenousperoxidaseactivity. 
 2.Phosphatebufferedsaline(PBS)solution.
 
 3.Distilledwater.
 
 4.Ethylalcohol
 
 5.Xylene
 
 6.Coverslips
 
 7.Bromodeoxyuridine(BrdU)
 
 8.Methanol
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