| CatalogueNumber | 2752 | 
| BrandFamily | Chemicon® 
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| TradeName |  | 
| Description | BrdUCellProliferationKit | 
| Overview | TheCHEMICONBrdUCellProliferationAssayKitisanon-isotopicassayfortheinvitroquantitativedetectionofnewlysynthesizedDNAofactivelyproliferatingcells. 
 TestPrinciple
 
 WithinCHEMICON"sBrdUCellProliferationAssayKit,mousemonoclonalantibodyisusedtodetecttheBrdUinasample.Afteradditionofgoatanti-mouseIgG-peroxidaseconjugatedsecondaryantibody,substrateandstopsolution,theamountofBrdUisdetermined.ThehighertheOD,thehighertheBrdUconcentrationinthesample.
 
 
 
 ForResearchUseOnly.Notforuseindiagnosticprocedures.
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| BackgroundInformation | Evaluationofcellcycleprogressionisessentialforinvestigationsinmanyscientificfields.Measurementof[3H]thymidineincorporationascellsenterSphasehasbeenatrADItionalmethodfordetectionofcellproliferation.Subsequentquantificationof[3H]thymidineisperformedbyscintillationcountingofautoradiography.Thistechnologyisslow,laborintensiveandhasseverallimitationsincludingthehandlinganddisposalofradioisotopesandthenecessityofexpensiveequipment. 
 Awellestablishedalternativeto[3H]thymidineuptakehasbeendemonstratedbynumerousresearchers.Inthesemethodsbromodeoxyuridine(BrdU),athymidineanalog,replaces[3H]thymidine.BrdUisincorporatedintonewlysynthesizedDNAstrandsofactivelyproliferatingcells.FollowingpartialdenaturationofdoublestrandedDNA,BrdUisdetectedimmunochemicallyallowingtheassessmentofthepopulationofcellswhicharesynthesizingDNA.
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| MaterialsRequiredbutNotDelivered | 
 1.Pipettors&tipscapableofaccuratelymeasuring2-1000μL
 
 2.Multi-channelorrepeatingPipettes
 
 3.Washbottleormultichanneldispensorforwashing
 
 4.2000mLgraduatedcylinder
 
 5.PBS(137mMNaCl,2.7mMKCl,4.3mMNa2HPO4-7H2O,1.4mMKH2PO4)
 
 6.DeionizedordistilledH2O.
 
 7.96-wellmicroplatereaderwith450-540nmor450-595nmdualwavelengthor450nmsinglewavelengthfilter
 
 8.Tissueculturemicroplate(96wellculturedish)
 
 9.Sterilereagenttroughs
 
 10.Microsyringefilter(0.2mm)
 
 11.Syringe
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